Difference between revisions of "Part:BBa K523014"
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====Experiment==== | ====Experiment==== | ||
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| + | We conducted two assays, comparing the activity of this part ''(bglX)'' with that of the exoglucanase cex (<partinfo>BBa_K118022</part>) on two different substrates: | ||
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| + | * 4-methylumbelliferyl-beta-D-glucuronide (MUG). This substrate mimics cellobiose but produces a fluorescent product when cleaved. | ||
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| + | * Something else. | ||
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| + | Our plates below shows the results of placing cell lysate and cell debris on an MUG plate. Present on both plates are: | ||
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| + | * Left side of plate: JM109 expressing this part, K523014 | ||
| + | * Right side of plate: JM109 expressing exoglucanase | ||
| + | * Bottom of plate: JM109 cells | ||
<center> | <center> | ||
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|[[Image:BglX-MuG.jpg|300px]] | |[[Image:BglX-MuG.jpg|300px]] | ||
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| + | |[[Image:Cex-MuC.jpg|300px]] | ||
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| − | |Foo. | + | |width="300px" valign="top"|Foo. |
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| + | |width="300px" valign="top"|Bar. | ||
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</center> | </center> | ||
Revision as of 13:14, 13 September 2011
Plac + LacZ + bglX
The E. coli periplasmic β-glucosidase gene bglX under the control of the lac promoter. The native ribosome binding site is present.
Usage and Biology
The BglX β-glucosidase is expected to be localised to the periplasm. It ought to be capable of breaking down cellobiose.
Experiment
We conducted two assays, comparing the activity of this part (bglX) with that of the exoglucanase cex (No part name specified with partinfo tag.