Difference between revisions of "Part:BBa K523014"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K523014 short</partinfo> | <partinfo>BBa_K523014 short</partinfo> | ||
| − | The ''E. coli'' periplasmic β-glucosidase gene bglX under the control of the lac promoter. The native ribosome binding site is present. | + | The ''E. coli'' periplasmic β-glucosidase gene ''bglX'' under the control of the lac promoter. The native ribosome binding site is present. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | The BglX β-glucosidase is expected to be localised to the periplasm. It ought to be capable of breaking down cellobiose. | ||
| + | |||
| + | ====Experiment==== | ||
| + | |||
| + | <center> | ||
| + | {| style="margin-top: 1em; margin-bottom: 1em;" | ||
| + | |- | ||
| + | |[[Image:BglX-MuG.jpg|300px]] | ||
| + | |- | ||
| + | |Foo. | ||
| + | |} | ||
| + | </center> | ||
<!-- --> | <!-- --> | ||
Revision as of 13:04, 13 September 2011
Plac + LacZ + bglX
The E. coli periplasmic β-glucosidase gene bglX under the control of the lac promoter. The native ribosome binding site is present.
Usage and Biology
The BglX β-glucosidase is expected to be localised to the periplasm. It ought to be capable of breaking down cellobiose.
Experiment
|
| Foo. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 607
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2266
Illegal AgeI site found at 2488
Illegal AgeI site found at 2677 - 1000COMPATIBLE WITH RFC[1000]