Difference between revisions of "Part:BBa K515100:Design"
(→Design Notes) |
|||
| Line 10: | Line 10: | ||
The coding regions have been optimised for B. subtilis and E. coli through the use of the program we have designed. | The coding regions have been optimised for B. subtilis and E. coli through the use of the program we have designed. | ||
| − | + | <html> | |
| − | The parts were assembled into pSB1C3 by CPEC assembly. | + | The parts were assembled into pSB1C3 by <a href="http://www.nature.com/nprot/journal/v6/n2/pdf/nprot.2010.181.pdf?WT.ec_id=NPROT-201102">CPEC assembly</a>. |
| − | + | </html> | |
===Source=== | ===Source=== | ||
Revision as of 10:17, 13 September 2011
IAA biosynthetic genes under control of the Pveg2 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 547
Illegal BamHI site found at 1492 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 254
Illegal NgoMIV site found at 2835 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An insulator sequence has been designed before each RBS to contain no homology with the vector, thereby avoiding recombination and allowing easier PCR removal of the individual parts from the device as well as promoter switching without influencing the RBS.
The coding regions have been optimised for B. subtilis and E. coli through the use of the program we have designed. The parts were assembled into pSB1C3 by CPEC assembly.
Source
Genomics sequence originating from P. savastanoi, synthesized by Eurofins.