Difference between revisions of "Part:BBa K515000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | <p>There is an insulator sequence before the RBS. This sequence has been specially designed to contain no homology with the vector, to avoid recombination and easier PCR of the parts. The insulator also alows switching of the promoter without influencing the RBS. </p> | + | <p>There is an insulator sequence before the RBS. This sequence has been specially designed to contain no homology with the vector, to avoid recombination and easier PCR of the parts. The insulator also alows switching of the promoter without influencing the RBS.</p> |
− | The coding region has been optimised for <i>B. subtilis</i> and <i>E. coli</i> through the use of the program we have designed. | + | <p>The coding region has been optimised for <i>B. subtilis</i> and <i>E. coli</i> through the use of the program we have designed.</p> |
− | The coding region originates from <i> P. savastanoi </i> and has previously been expressed in <i>E. coli</i> | + | <p>The coding region originates from <i> P. savastanoi </i> and has previously been expressed in <i>E. coli</i> [1] </p> |
===Source=== | ===Source=== | ||
− | + | <p>Genomics sequence originating from <i>P. savastanoi</i>, synthesized by Eurofins.</p> | |
===References=== | ===References=== | ||
+ | |||
+ | <p>[1] Palm C. et al., 1988. Cotranscription of Genes Encoding Indoleacetic Acid Production in <i>Pseudomonas syringae</i> subsp. <i>savastanoi</i>. <i>Journal of Bacteriology</i>, 172(2), pp.1002-1009. </p> |
Latest revision as of 15:10, 12 September 2011
IaaM - tryptophan-2-mono-oxygenase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 450
Illegal BamHI site found at 1395 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 157
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is an insulator sequence before the RBS. This sequence has been specially designed to contain no homology with the vector, to avoid recombination and easier PCR of the parts. The insulator also alows switching of the promoter without influencing the RBS.
The coding region has been optimised for B. subtilis and E. coli through the use of the program we have designed.
The coding region originates from P. savastanoi and has previously been expressed in E. coli [1]
Source
Genomics sequence originating from P. savastanoi, synthesized by Eurofins.
References
[1] Palm C. et al., 1988. Cotranscription of Genes Encoding Indoleacetic Acid Production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 172(2), pp.1002-1009.