Difference between revisions of "Part:BBa K515100"
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<b>Compatability</b>
 
<b>Compatability</b>
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<p>Chassis: This device has been tested in <i>E. coli</i> strain DH5α as a construct assembled with the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone vector. </p>  
 
<p>Chassis: This device has been tested in <i>E. coli</i> strain DH5α as a construct assembled with the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone vector. </p>  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:08, 12 September 2011

IAA biosynthetic genes under control of the Pveg2 promoter

The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) BBa_K515000, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) BBa_K515001 . There are several different pathways that produce indole-3-acetic acid. IaaM and IaaH originate from P.savastanoi and have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]

Compatability

Chassis: This device has been tested in E. coli strain DH5α as a construct assembled with the pSB1C3 backbone vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
    Illegal BamHI site found at 1492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 254
    Illegal NgoMIV site found at 2835
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 171(2), pp.1002-1009.</p>