Difference between revisions of "Part:BBa K515000"
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<partinfo>BBa_K515000 short</partinfo>
 
<partinfo>BBa_K515000 short</partinfo>
  
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IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. There are several different pathways to produce indole-3-acetic acid. [1] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, <i>Psuedomonas savastanoi</i> to produce indole-3-acetic acid in <i>E. coli</i> </p>
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IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, <i> Pseudomonas savastanoi </i> </p>
  
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<p> tryptophan --> indole acetamide --> indole-3-acetic acid </p>
 
  
  

Revision as of 13:56, 12 September 2011

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. This auxin producing pathway originates from the plant pathogen, Pseudomonas savastanoi </p>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448