Difference between revisions of "Part:BBa K515100"
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<partinfo>BBa_K515100 short</partinfo> | <partinfo>BBa_K515100 short</partinfo> | ||
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− | <p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) <a href="https://parts.igem.org/Part:BBa_K515000">BBa_K515000</a>, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) <a href="https://parts.igem.org/Part:BBa_K515001">BBa_K515001</a> . There are several different pathways that produce indole-3-acetic acid. | + | <p> The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) <a href="https://parts.igem.org/Part:BBa_K515000">BBa_K515000</a>, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) <a href="https://parts.igem.org/Part:BBa_K515001">BBa_K515001</a> . There are several different pathways that produce indole-3-acetic acid. IaaM and IaaH originate from <i>P.savastanoi</i> and have been expressed in <i>E. coli</i> previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]</p> |
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<b>Compatability</b> | <b>Compatability</b> |
Revision as of 13:54, 12 September 2011
IAA biosynthetic genes under control of the Pveg2 promoter
The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) BBa_K515000, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) BBa_K515001 . There are several different pathways that produce indole-3-acetic acid. IaaM and IaaH originate from P.savastanoi and have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]
CompatabilityChassis: This device has been tested in E. coli strain Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 547
Illegal BamHI site found at 1492 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 254
Illegal NgoMIV site found at 2835 - 1000COMPATIBLE WITH RFC[1000]