Difference between revisions of "Part:BBa K678002"
Line 4: Line 4:
 
Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)
 
Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below).
 +
https://static.igem.org/mediawiki/igem.org/c/ce/26.08.2011_12-1%2B16_034.tif
 +
 +
This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.
 +
https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 11:57, 12 September 2011

eGFP-PTS1 for mammalian cells

Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)

Usage and Biology

We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below). https://static.igem.org/mediawiki/igem.org/c/ce/26.08.2011_12-1%2B16_034.tif

This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein. https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]