Difference between revisions of "Part:BBa K606025"

Revision as of 10:29, 12 September 2011

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed by a 10kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 631
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K606025 short</partinfo>
-YFP:tetR protein fusion. <br>
 YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO). Using the tetO array  (<partinfo>BBa_K606026</partinfo>) composed by a 10kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.<br> <br>Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)