Difference between revisions of "Part:BBa K515102:Experience"
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<p> Two tests were performed to observe chemotactic response over a large range of chemoattractant concentration and to observe differences in much finer milimolar ranges of attractant concentration.</p>
 
<p> Two tests were performed to observe chemotactic response over a large range of chemoattractant concentration and to observe differences in much finer milimolar ranges of attractant concentration.</p>
 
<p> Table 1: Ranges of attractant concentration tested.</p>
 
<p> Table 1: Ranges of attractant concentration tested.</p>
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  concentration</span></p>
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<p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:
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 +
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 +
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 +
<p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:
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===User Reviews===
 
===User Reviews===

Revision as of 17:11, 10 September 2011

Characterisation

This part (BBa K515102) has been characterised qualitatively and quantitatively for chemotactic response towards L (-) malic acid. The tests were performed across a range of different attractant concentrations to observe optimal functioning of the part. Qualitative tests were performed using semi solid agar assays, quantitative analysis was performed using high - throughput capillary assay with cell count for each capillary evaluated using FACS. Behavioral analysis of E. coli expressing this part was done using Wide - field microscopy, and manual tracking was used to evaluate tactic response. Negative control used was E. coli 5α strain with a selectable marker for ampicillin and kanamycin. As positive control chemotactic response of E. coli 5α strain to attractant L (-) serine was tested, as serine is compatible with endogenous chemotaxis system of E. coli 5α strain.

Qualitative assay

Two tests were performed to observe chemotactic response over a large range of chemoattractant concentration and to observe differences in much finer milimolar ranges of attractant concentration.

Table 1: Ranges of attractant concentration tested.

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Attractant concentration

Test 1

0 M

10-5 M

10-4 M

10-3 M

10-2 M

10-1 M

Test 2

0 mM

5 mM

10 mM

15 mM

20 mM

25 mM


User Reviews

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