Difference between revisions of "Part:BBa K515000"
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<partinfo>BBa_K515000 short</partinfo>
 
<partinfo>BBa_K515000 short</partinfo>
  
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IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, <i>Psuedomonas savastanoi</i> which produces and secretes indole-3-acetic acid (auxin).[1]
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IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.[1] There are several different pathways to produce indole-3-acetic acid. [2] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, <i>Psuedomonas savastanoi</i> to produce indole-3-acetic acid in <i>E. coli</i> </p>
  
 
<p> tryptophan --> indole acetamide --> indole-3-acetic acid </p>
 
<p> tryptophan --> indole acetamide --> indole-3-acetic acid </p>
  
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The sequence has been codon optimised for <i>E. coli</i> and <i>B. subtilis</i> and is under control of the pVEG promoter with an RBS also suitable for <i>E. coli</i> and <i>B. subtilis</i>.
 
  
 
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<h2>References</h2>
 
<h2>References</h2>
 
[1]http://ecocyc.org/META/NEW-IMAGE?type=GENE&object=G-5821
 
[1]http://ecocyc.org/META/NEW-IMAGE?type=GENE&object=G-5821
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[2]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. <i>Federation of European Microbiological Societies Microbiology Reviews </i>, 31, pp.425–448 </p>

Revision as of 14:51, 10 September 2011

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.[1] There are several different pathways to produce indole-3-acetic acid. [2] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, Psuedomonas savastanoi to produce indole-3-acetic acid in E. coli </p>

tryptophan --> indole acetamide --> indole-3-acetic acid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]http://ecocyc.org/META/NEW-IMAGE?type=GENE&object=G-5821 [2]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448 </p>