Difference between revisions of "Part:BBa K515000"

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IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, <i>Psuedomonas savastanoi</i> which produces and secretes indole-3-acetic acid (auxin).[1]  
 
IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, <i>Psuedomonas savastanoi</i> which produces and secretes indole-3-acetic acid (auxin).[1]  
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<p> tryptophan --> indole acetamide --> indole-3-acetic acid </p>
  
 
The sequence has been codon optimised for <i>E. coli</i> and <i>B. subtilis</i> and is under control of the pVEG promoter with an RBS also suitable for <i>E. coli</i> and <i>B. subtilis</i>.
 
The sequence has been codon optimised for <i>E. coli</i> and <i>B. subtilis</i> and is under control of the pVEG promoter with an RBS also suitable for <i>E. coli</i> and <i>B. subtilis</i>.

Revision as of 13:41, 10 September 2011

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, Psuedomonas savastanoi which produces and secretes indole-3-acetic acid (auxin).[1]

tryptophan --> indole acetamide --> indole-3-acetic acid

The sequence has been codon optimised for E. coli and B. subtilis and is under control of the pVEG promoter with an RBS also suitable for E. coli and B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]http://ecocyc.org/META/NEW-IMAGE?type=GENE&object=G-5821