Difference between revisions of "Part:BBa K515102"
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<b>Description</b> | <b>Description</b> | ||
− | <p>Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).<p> | + | <p>Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).</p> |
<b>Compatibility</b> | <b>Compatibility</b> | ||
− | <p>Device has been tested in <i>E. coli | + | <p>Device has been tested in <i>E. coli DH5α strain</i>, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of <i>E. coli<i>.</p> |
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Revision as of 11:10, 10 September 2011
J23100 promoter - PA2652
Description
Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).
Compatibility
Device has been tested in E. coli DH5α strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of E. coli<i>.</p>
Sequence and Features
Illegal NheI site found at 30
Illegal NgoMIV site found at 812
Illegal AgeI site found at 118