Difference between revisions of "Part:BBa K515102"
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<partinfo>BBa_K515102 short</partinfo>
 
<partinfo>BBa_K515102 short</partinfo>
  
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A composite part comprising of a J23100 promoter and PA2652 translational unit encoding transmembrane chemoreceptor
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<b>Description</b>
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<p>Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).<p>
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<b>Compatibility</b>
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<p>Device has been tested in <i>E. coli 5α strain</i>
  
 
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Revision as of 11:04, 10 September 2011

J23100 promoter - PA2652

Description

Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).<p> Compatibility <p>Device has been tested in E. coli 5α strain Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 812
    Illegal AgeI site found at 118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1019