Difference between revisions of "Part:BBa K644000:Design"
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===Design Notes=== | ===Design Notes=== | ||
Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3). | Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3). | ||
| − | + | ||
| + | - We also mutated the EcoRI endonuclease site to make E-cadherin compatible for the the Parts Registry. | ||
| + | |||
===Source=== | ===Source=== | ||
Latest revision as of 18:37, 9 September 2011
Extracellular Domain of E-Cadherin (Mouse)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 285
Illegal BamHI site found at 361
Illegal BamHI site found at 477
Illegal BamHI site found at 1401
Illegal BamHI site found at 1703
Illegal XhoI site found at 1085 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 405
Illegal BsaI site found at 947
Design Notes
Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3).
- We also mutated the EcoRI endonuclease site to make E-cadherin compatible for the the Parts Registry.
Source
Mus musculus (mouse)