Difference between revisions of "Part:BBa K644000:Design"

 
 
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<partinfo>BBa_K644000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3).  
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Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3).
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- We also mutated the EcoRI endonuclease site to make E-cadherin compatible for the the Parts Registry.
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===Source===
 
===Source===

Latest revision as of 18:37, 9 September 2011

Extracellular Domain of E-Cadherin (Mouse)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
    Illegal BamHI site found at 361
    Illegal BamHI site found at 477
    Illegal BamHI site found at 1401
    Illegal BamHI site found at 1703
    Illegal XhoI site found at 1085
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 405
    Illegal BsaI site found at 947


Design Notes

Our E-cadherin only contains the extracellular gene sequence because we only needed the extracellular region for our project. Since the E-cadherin gene sequence has an EcoRI restriction endonuclease site, we had to use the Gibson Assembly to clone our E-cadherin gene into the BioBrick plasmid (pSB1C3).

- We also mutated the EcoRI endonuclease site to make E-cadherin compatible for the the Parts Registry.


Source

Mus musculus (mouse)

References