Difference between revisions of "Part:BBa K523000:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K523000 short</partinfo>
 
<partinfo>BBa_K523000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.
 
  
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Made with PCR from <partinfo>BBa_J33207</partinfo> using primers:
  
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* '''Forward:''' gcttctagagatctgctggggcaaaccagcg
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* '''Reverse:''' a standard reverse primer for amplifying BioBricks
  
 
===Source===
 
===Source===
  
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Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
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Part <partinfo>BBa_J33207</partinfo> was originally amplified from ''E. coli'' BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
  
 
===References===
 
===References===

Latest revision as of 16:03, 7 September 2011

Plac + lacZ; with BglII site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with PCR from BBa_J33207 using primers:

  • Forward: gcttctagagatctgctggggcaaaccagcg
  • Reverse: a standard reverse primer for amplifying BioBricks

Source

Part BBa_J33207 was originally amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).

References