Difference between revisions of "Part:BBa K523000:Design"
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Made with PCR from <partinfo>BBa_J33207</partinfo> using primers: | Made with PCR from <partinfo>BBa_J33207</partinfo> using primers: | ||
| − | * Forward: gcttctagagatctgctggggcaaaccagcg | + | * '''Forward:''' gcttctagagatctgctggggcaaaccagcg |
| − | * Reverse: a standard reverse primer for amplifying BioBricks | + | * '''Reverse:''' a standard reverse primer for amplifying BioBricks |
===Source=== | ===Source=== | ||
Revision as of 16:02, 7 September 2011
Plac + lacZ; with BglII site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made with PCR from BBa_J33207 using primers:
- Forward: gcttctagagatctgctggggcaaaccagcg
- Reverse: a standard reverse primer for amplifying BioBricks
Source
Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).