Difference between revisions of "Part:BBa K523013"
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<partinfo>BBa_K523013 short</partinfo> | <partinfo>BBa_K523013 short</partinfo> | ||
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Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter. | Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Cells expressing this construct are shown on the right. The INP should carry the EYFP to the outer membrane of ''E. coli''. Our imaging technology was not good enough to confirm this. | Cells expressing this construct are shown on the right. The INP should carry the EYFP to the outer membrane of ''E. coli''. Our imaging technology was not good enough to confirm this. | ||
Revision as of 09:48, 6 September 2011
Plac + INP-EYFP
Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter.
Constructed using the BioSandwich protocol of Edinburgh 2011.
Usage and Biology
Cells expressing this construct are shown on the right. The INP should carry the EYFP to the outer membrane of E. coli. Our imaging technology was not good enough to confirm this.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
- 1000COMPATIBLE WITH RFC[1000]