Difference between revisions of "Part:BBa K523002"

(→‎Usage and Biology)
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The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
 
The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
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The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 if and when site-specific mutagenesis has been carried out. Alternatively, since this site is contained within a localisation signal, we might remake the part minus that signal.
 
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:02, 23 August 2011

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli β-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

The product protein is believed to be periplasmic. Beta-glucosidase cleaves β(1→4) bonds, i.e. those found in the disaccharide cellobiose.

The SignalP program predicts that a 20 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
    Illegal AgeI site found at 1880
    Illegal AgeI site found at 2069
  • 1000
    COMPATIBLE WITH RFC[1000]