Difference between revisions of "Production/Competent Cells"
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| − | <tr><th>Batch<th>Strain<th>Maker<th>Date Started<th>Temp, RPM<th>Incubation (hr)<th>Culture Volume<th>Culture OD (600nm) | + | <!-- Table Header (Titles)--!> |
| − | <th>Competent OD (600nm)<th>Cell Count (AVG)<th>Efficiency<th>250ul Aliquots<th>500ul Aliquots<th>1ml Aliquots</tr> | + | <tr><th>Batch <th>Strain <th>Maker <th>Date Started <th>Temp, RPM <th>Incubation (hr) <th>Culture Volume <th>Culture OD (600nm) <th>Competent OD (600nm) <th>Cell Count (AVG) <th>Efficiency <th>250ul Aliquots <th>500ul Aliquots <th>1ml Aliquots <th>96w Plate</tr> |
| − | <tr><td>001<td>NEB 10b<td>VS+KN<td>8/17/11<td>21C, 250rpm<td>16<td>500ml<td>0.4 | + | |
| − | <td>1.35<td>67,88,112 (89)<td>1.335*10^8<td>20<td>20<td>10 | + | <!-- Expected Values --!> |
| + | <tr style="background-color:#C0C0C0;"><td>Expected Values<td><td><td><td>20C<td>16<td><td>0.3 or lower | ||
| + | <td>1 - 1.5<td>100 - 400<td>5*10^8 - 5*10^9<td><td><td><td></tr> | ||
| + | |||
| + | <!-- Batch 001 Data Starts--!> | ||
| + | <tr><td>001 <td>NEB 10b <td>VS+KN <td>8/17/11 <td>21C, 250rpm <td>16 <td>500ml <td>0.4 <td>1.35 <td>67,88,112 (89) <td>1.335*10^8 <td>20 <td>20 <td>10 <td>0</tr> | ||
| + | <!-- Batch 001 Data Ends--!> | ||
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==Batch Notes== | ==Batch Notes== | ||
===001=== | ===001=== | ||
| − | This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch | + | This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch! And a resounding success! |
| + | |||
| + | The efficiency test/test transformation used a single 250ul aliquot (randomly chosen). Three transformations were done using our standard template and protocol. '''Important:''' In following productions we will test the first and last of the aliquots. | ||
Revision as of 18:32, 19 August 2011
Batch Summary
Use this page to keep track of competent cell production. See the competent cell protocol for more information.
| Batch | Strain | Maker | Date Started | Temp, RPM | Incubation (hr) | Culture Volume | Culture OD (600nm) | Competent OD (600nm) | Cell Count (AVG) | Efficiency | 250ul Aliquots | 500ul Aliquots | 1ml Aliquots | 96w Plate |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Expected Values | 20C | 16 | 0.3 or lower | 1 - 1.5 | 100 - 400 | 5*10^8 - 5*10^9 | ||||||||
| 001 | NEB 10b | VS+KN | 8/17/11 | 21C, 250rpm | 16 | 500ml | 0.4 | 1.35 | 67,88,112 (89) | 1.335*10^8 | 20 | 20 | 10 | 0 |
Batch Notes
001
This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch! And a resounding success!
The efficiency test/test transformation used a single 250ul aliquot (randomly chosen). Three transformations were done using our standard template and protocol. Important: In following productions we will test the first and last of the aliquots.