Difference between revisions of "Part:BBa K349101:Experience"
(→User Reviews) |
|||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K349101=== | ===Applications of BBa_K349101=== | ||
| + | Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K349101 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K349101 StartReviews</partinfo> | ||
| + | Karina Arnesen- Alberta iGEM 2010 team- I've noticed that the B' end was designed in such a way that the stop codon is incorrectly placed, or non-existent when used with BA parts using the BioBytes v2.0 assembly method. | ||
<!-- Template for a user review | <!-- Template for a user review | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
|- | |- | ||
|width='10%'| | |width='10%'| | ||
| + | |||
<partinfo>BBa_K349101 AddReview number</partinfo> | <partinfo>BBa_K349101 AddReview number</partinfo> | ||
<I>Username</I> | <I>Username</I> | ||
Latest revision as of 19:53, 11 August 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K349101
Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method.
User Reviews
UNIQ06fafad6ed11c067-partinfo-00000000-QINU Karina Arnesen- Alberta iGEM 2010 team- I've noticed that the B' end was designed in such a way that the stop codon is incorrectly placed, or non-existent when used with BA parts using the BioBytes v2.0 assembly method. UNIQ06fafad6ed11c067-partinfo-00000001-QINU