Difference between revisions of "Part:BBa K523010"

Revision as of 08:58, 11 August 2011

β-glucosidase for BioSandwich

This is a modified version of BBa_K392008 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

We discovered late on that the first 220 or so bases are not coding; the part needs to be remade.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 863
Illegal XhoI site found at 1097
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 350
Illegal AgeI site found at 246
Illegal AgeI site found at 441
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 223

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K523010 short</partinfo>
@@ Line 6: / Line 5: @@
 BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
+'''We discovered late on that the first 220 or so bases are not coding; the part needs to be remade.'''
 <!-- Add more about the biology of this part here