Difference between revisions of "Part:BBa K523002"
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<partinfo>BBa_K523002 short</partinfo> | <partinfo>BBa_K523002 short</partinfo> | ||
| − | This is the ''E. coli'' beta-glucosidase gene ''bglX''. The part contains the native Ribosome Binding Site. | + | This is the ''E. coli'' β-glucosidase gene ''bglX''. The part contains the native Ribosome Binding Site. |
The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | ||
Revision as of 12:21, 29 July 2011
RBS + bglX (E. coli perisplasmic β-glucosidase)
This is the E. coli β-glucosidase gene bglX. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 if and when site-specific mutagenesis has been carried out. Alternatively, since this site is contained within a localisation signal, we might remake the part minus that signal.
Usage and Biology
The product protein is believed to be periplasmic. Beta-glucosidase cleaves Β(1→4) bonds, i.e. those found in the disaccharide cellobiose.
The SignalP program predicts that a 20 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1658
Illegal AgeI site found at 1880
Illegal AgeI site found at 2069 - 1000COMPATIBLE WITH RFC[1000]