Difference between revisions of "Part:BBa K523003"

Revision as of 10:10, 29 July 2011

malS for BioSandwich

This is a version of BBa_K523001 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.

There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.

The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1204
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 340
Illegal AgeI site found at 1505
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K523003 short</partinfo>
@@ Line 5: / Line 4: @@
 This is a version of <partinfo>BBa_K523001</partinfo> but with no RBS and no stop codon.
-There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.
+There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
+There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
+The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
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