Difference between revisions of "Part:BBa K523002"

Revision as of 18:12, 19 July 2011

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 if and when site-specific mutagenesis has been carried out. Alternatively, since this site is contained within a localisation signal, we might remake the part minus that signal.

Usage and Biology

The product protein is believed to be periplasmic. Beta-glucosidase cleaves Β(1→4) bonds, i.e. those found in the disaccharide cellobiose.

The SignalP program predicts that a 20 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.

Sequence and Features