Difference between revisions of "Part:BBa K523001"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 10: | Line 10: | ||
The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in ''E. coli'' presumably involves degrading shorter glucose chains. | The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in ''E. coli'' presumably involves degrading shorter glucose chains. | ||
| + | The SignalP program predicts that a 17 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 18:05, 19 July 2011
RBS + malS (E. coli periplasmic α-amylase)
This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
Usage and Biology
The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in E. coli presumably involves degrading shorter glucose chains.
The SignalP program predicts that a 17 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1233
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 369
Illegal AgeI site found at 1534 - 1000COMPATIBLE WITH RFC[1000]