Difference between revisions of "Part:BBa K523002"
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The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site. | ||
| − | This part will be edited to be compliant with RFC10 | + | The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 after site-specific mutagenesis has been carried out. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:26, 19 July 2011
RBS + bglX (E. coli perisplasmic β-glucosidase)
This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 after site-specific mutagenesis has been carried out.
Usage and Biology
The product protein is believed to be periplasmic. A beta-glucosidase cleaves Β1-4 bonds, i.e. those found in the disaccharide cellobiose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1658
Illegal AgeI site found at 1880
Illegal AgeI site found at 2069 - 1000COMPATIBLE WITH RFC[1000]