Difference between revisions of "Part:BBa K523002"

 
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<partinfo>BBa_K523002 short</partinfo>
 
<partinfo>BBa_K523002 short</partinfo>
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The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
 
The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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The product protein is believed to be periplasmic.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K523002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K523002 SequenceAndFeatures</partinfo>

Revision as of 15:16, 19 July 2011

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

The product protein is believed to be periplasmic.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
    Illegal AgeI site found at 1880
    Illegal AgeI site found at 2069
  • 1000
    COMPATIBLE WITH RFC[1000]