Difference between revisions of "Part:BBa K124017:Experience"

Latest revision as of 08:58, 14 July 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K124017

User Reviews

Caltech 2010: It appears that this part's sequence has been mixed up with BBa_K124014. The gel data on each part page appears to corroborate this mix-up.

Sequencing by the 2011 iGEM Team Freiburg supports the finding of the Caltech team 2011. Additionally following errors compared to BBa_K124014 were found:

The wrong prefix was used what will finally lead to a RBS-ATG distance of 8 instead of 6 nucleotides.

Two single nucleotide exchanges could be found.

The overall sequence resembles the wildtype phage sequence and not the artificially constructed sequence submitted for BBa_K124014.

UNIQ470123bcf34adff9-partinfo-00000003-QINU UNIQ470123bcf34adff9-partinfo-00000004-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
@@ Line 7: / Line 6: @@
 ===User Reviews===
+Caltech 2010: It appears that this part's sequence has been mixed up with <partinfo>K124014</partinfo>. The gel data on each part page appears to corroborate this mix-up.
+<BR>
+<HR>
+Sequencing by the 2011 iGEM Team Freiburg supports the finding of the Caltech team 2011. Additionally following errors compared to <partinfo>K124014</partinfo> were found:
+*The wrong prefix was used what will finally lead to a RBS-ATG distance of 8 instead of 6 nucleotides.
+*Two single nucleotide exchanges could be found.
+*The overall sequence resembles the wildtype phage sequence and not the artificially constructed sequence submitted for <partinfo>K124014</partinfo>.
+<HR>
 <!-- DON'T DELETE --><partinfo>BBa_K124017 StartReviews</partinfo>
 <!-- Template for a user review