Difference between revisions of "Part:BBa K648005:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K648005 short</partinfo> | <partinfo>BBa_K648005 short</partinfo> | ||
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Forward (57.78 degrees) | Forward (57.78 degrees) | ||
TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtggttctggtACC | TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtggttctggtACC | ||
| + | |||
Reverse (57.78 degrees) | Reverse (57.78 degrees) | ||
AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccagaaccaccG | AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccagaaccaccG | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 16:22, 3 July 2011
Short Fusion Protein Linker: GGSG with standard 25 prefix/suffix
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.
This part was synthesized through PCR oligo synthesis methods using the following primers:
Forward (57.78 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtggttctggtACC
Reverse (57.78 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccagaaccaccG
Source
Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z)
Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529