Difference between revisions of "Part:BBa K523000:Design"

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===Source===
 
===Source===
  
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Based on part <partinfo>BBa_J33207</partinfo>, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
+
Based on part <partinfo>BBa_J33207</partinfo>, which was amplified from ''E. coli'' BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).
  
 
===References===
 
===References===

Revision as of 21:22, 28 June 2011

Plac + lacZ; with BglII site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.


Source

Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).

References