Difference between revisions of "Part:BBa K123003:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | <partinfo>BBa_K123003 AddReview 1</partinfo> | ||
| + | <I>jbrown2 ([http://2011.igem.org/Team:Caltech Caltech iGEM team 2011])</I> | ||
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| + | We ran the DNA sequence through the ExPASy translator with the following result: | ||
| + | |||
| + | MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA | ||
| + | YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP | ||
| + | FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA | ||
| + | KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA | ||
| + | CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR | ||
| + | RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT | ||
| + | WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC | ||
| + | CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL | ||
| + | WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- | ||
| + | NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR | ||
| + | KVSRRPLPV | ||
| + | |||
| + | It looks like there's an intron in the coding sequence. This should be the correct sequence: | ||
| + | |||
| + | >gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] | ||
| + | MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA | ||
| + | QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT | ||
| + | VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK | ||
| + | RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV | ||
| + | GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA | ||
| + | DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG | ||
| + | MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM | ||
| + | AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV | ||
| + | EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV | ||
| + | |||
| + | |||
| + | |}; | ||
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Revision as of 17:58, 21 June 2011
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UNIQ5ee8047e942bcb19-partinfo-00000000-QINU
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jbrown2 ([http://2011.igem.org/Team:Caltech Caltech iGEM team 2011]) |
We ran the DNA sequence through the ExPASy translator with the following result: MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR KVSRRPLPV It looks like there's an intron in the coding sequence. This should be the correct sequence: >gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV
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UNIQ5ee8047e942bcb19-partinfo-00000002-QINU