Difference between revisions of "Part:BBa I20531"
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All rights not already held by others are '''PUBLIC DOMAIN''' as per [http://biobricks.org/bpa/contributions/2/ BPA Contributor Agreement #2] in compliance with [http://rph.stanford.edu/5-1.html Section 5.1.A.3] of the Stanford Research Policy Handbook. | All rights not already held by others are '''PUBLIC DOMAIN''' as per [http://biobricks.org/bpa/contributions/2/ BPA Contributor Agreement #2] in compliance with [http://rph.stanford.edu/5-1.html Section 5.1.A.3] of the Stanford Research Policy Handbook. | ||
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=== Acknowledgement === | === Acknowledgement === |
Latest revision as of 17:25, 21 June 2011
CDS for phage k1-5 RNA polymerase
Coding sequence for K1-5 RNA polymerase. Useful for orthogonal transcription. Cognate consensus promoter encoded by BBa_I20530.
Property Rights
All rights not already held by others are PUBLIC DOMAIN as per [http://biobricks.org/bpa/contributions/2/ BPA Contributor Agreement #2] in compliance with [http://rph.stanford.edu/5-1.html Section 5.1.A.3] of the Stanford Research Policy Handbook.
[http://biobricks.org/bpa ]
Acknowledgement
[http://www.biosci.utexas.edu/mgm/People/Faculty/profiles/?id=1707 Prof. Ian J. Molineux] of UT Austin suggested the K1-5 RNA polymerase system as a orthogonal transcription system distinct from T3, T7, SP6, et al.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 658
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1467
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 1000COMPATIBLE WITH RFC[1000]