Difference between revisions of "Part:BBa I20531:Design"
(→Design Notes) |
(→References) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 9: | Line 9: | ||
===Source=== | ===Source=== | ||
| − | + | Nucleotide sequence of the annotated K1-5 genome as per Genbank AY370674.1 | |
| − | + | ||
===References=== | ===References=== | ||
| + | Genomic Analysis of Bacteriophages SP6 and K1-5, an Estranged Subgroup of the T7 Supergroup. | ||
| + | Journal of Molecular Biology | ||
| + | Volume 335, Issue 5, 30 January 2004, Pages 1151-1171 | ||
| + | doi:10.1016/j.jmb.2003.11.035 | ||
Latest revision as of 17:54, 17 June 2011
CDS for phage k1-5 RNA polymerase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 658
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1467
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 951
Illegal SpeI site found at 658 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Native DNA sequence has not here been refined to support compatibility with any existing physical DNA assembly protocol.
Source
Nucleotide sequence of the annotated K1-5 genome as per Genbank AY370674.1
References
Genomic Analysis of Bacteriophages SP6 and K1-5, an Estranged Subgroup of the T7 Supergroup. Journal of Molecular Biology Volume 335, Issue 5, 30 January 2004, Pages 1151-1171 doi:10.1016/j.jmb.2003.11.035