Difference between revisions of "Part:BBa M36706:Design"

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===Design Notes===
 
===Design Notes===
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Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included.
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===Source===
 
===Source===

Latest revision as of 21:11, 5 May 2011

Gene encoding IclR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included.

Source

This coding sequence was obtained from genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=c

References