Difference between revisions of "Part:BBa K176011:Design"

 
(→‎Design Notes)
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176011 short</partinfo>
 
<partinfo>BBa_K176011 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Report of pCon 1.0(J23100)
 
  
 +
Primers:
 +
Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix.
 +
Sequences provided by Gingko BioWorks.
 +
 +
Fwd: GCTTCTAGAGATACATGAAGATGCACTTttgacggctagctcagtcctagg
  
 +
Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg
  
 
===Source===
 
===Source===

Latest revision as of 03:49, 30 December 2010

pCon 1.00(J23100)->RBS+GFP+T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAAGATGCACTTttgacggctagctcagtcctagg

Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg

Source

J23100 I13504

References