Difference between revisions of "Part:BBa I13522:Design"
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<partinfo>BBa_I13522 short</partinfo> | <partinfo>BBa_I13522 short</partinfo> | ||
<partinfo>BBa_I13522 SequenceAndFeatures</partinfo> | <partinfo>BBa_I13522 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| + | Primers: | ||
| + | Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. | ||
| + | Sequences provided by Gingko BioWorks. | ||
| + | Fwd: GCTTCTAGAGATACATGAAGATGCACTTtccctatcagtgatagagattgacatcc | ||
| + | |||
| + | Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg | ||
===Source=== | ===Source=== | ||
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| − | |||
| − | + | ===References=== | |
| − | === | + | |
Latest revision as of 03:47, 30 December 2010
pTet GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAAGATGCACTTtccctatcagtgatagagattgacatcc
Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg