Difference between revisions of "Part:BBa J04450:Design"

 
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__NOTOC__
 
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<partinfo>BBa_J04450 short</partinfo>
 
<partinfo>BBa_J04450 short</partinfo>
  
 
<partinfo>BBa_J04450 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J04450 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
  
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Primers:
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Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix.
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Sequences provided by Gingko BioWorks.
  
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fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc
  
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===Source===
 
  
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--What is the source of this part?--
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rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
  
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===References===
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===Source===
  
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Davidson Synth-Aces
  
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===Files===
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===References===

Latest revision as of 03:43, 30 December 2010

RFP Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc


rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

Davidson Synth-Aces

References