Difference between revisions of "Part:BBa J04450:Design"

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Primers:
 
Primers:
−
Note: EcoRI sites removed. Must use XP.  
+
Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix.
 
Sequences provided by Gingko BioWorks.
 
Sequences provided by Gingko BioWorks.
  
 
fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc
 
fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc
 +
 +
 
rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
 
rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
  

Latest revision as of 03:43, 30 December 2010

RFP Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

fwd:GCTTCTAGAGATACATGAACATGCAATACcaatacgcaaaccgcctctccc


rv:CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

Davidson Synth-Aces

References