Difference between revisions of "Part:BBa J70603:Design"

 
(→‎Design Notes)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70603 short</partinfo>
 
<partinfo>BBa_J70603 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Designed
+
Primer Design Notes:
  
 +
Forward primer: gtttcttcgaattcgcggccgcttctagatggagaaccgtgagccaccac
 +
l:50
 +
P(g/b):g
 +
Ho(max#):-104.84
 +
Het(max#):-112.52
 +
Tm:71.1
 +
GC(%): 56
  
 +
Reverse primer: gtttcttcctgcagcggccgctactagtattattagcgcttggccgcgaaaac
 +
l:53
 +
P(g/b):b
 +
Ho(max#):-112.52
 +
Het(max#):-112.52
 +
Tm:70.6
 +
GC(%):52.8
  
 
===Source===
 
===Source===

Latest revision as of 17:39, 19 November 2010

VioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatggagaaccgtgagccaccac l:50 P(g/b):g Ho(max#):-104.84 Het(max#):-112.52 Tm:71.1 GC(%): 56

Reverse primer: gtttcttcctgcagcggccgctactagtattattagcgcttggccgcgaaaac l:53 P(g/b):b Ho(max#):-112.52 Het(max#):-112.52 Tm:70.6 GC(%):52.8

Source

Designed

References