Difference between revisions of "Part:BBa J70600:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70600 short</partinfo>
 
<partinfo>BBa_J70600 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
designed
+
Primer Design Notes:
  
 +
Forward primer: gtttcttcgaattcgcggccgcttctagatgagcattctggatttcccgcgtatc
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l: 55
 +
P(g/b): g
 +
Ho(max#): -114.97
 +
Het(max#): -114.97
 +
Tm: 70.0
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GC(%): 50.9
  
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Reverse primer: gtttcttcctgcagcggccgctactagtattattaggcctcgcggctcagtttg
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l: 54
 +
P(g/b): b
 +
Ho(max#): -111.67
 +
Het(max#): -114.97
 +
Tm: 70.7
 +
GC(%): 53.7
  
 
===Source===
 
===Source===

Latest revision as of 17:38, 19 November 2010

VioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2523
    Illegal AgeI site found at 2719
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgagcattctggatttcccgcgtatc l: 55 P(g/b): g Ho(max#): -114.97 Het(max#): -114.97 Tm: 70.0 GC(%): 50.9

Reverse primer: gtttcttcctgcagcggccgctactagtattattaggcctcgcggctcagtttg l: 54 P(g/b): b Ho(max#): -111.67 Het(max#): -114.97 Tm: 70.7 GC(%): 53.7

Source

designed

References