Difference between revisions of "Part:BBa J70638:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70638 short</partinfo>
 
<partinfo>BBa_J70638 short</partinfo>
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derived from K274002  
 
derived from K274002  
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Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC
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Suffix: TBD
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J70638 A Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC
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 +
J70638 B Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG
  
 
===References===
 
===References===

Latest revision as of 06:44, 19 November 2010

rbs VioA rbs VioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there was a sequence between the stop codon of vioB and rbs of vioC in original part


Source

derived from K274002

J70638 A Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC

J70638 B Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG

References