Difference between revisions of "Part:BBa J70638:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70638 short</partinfo> | <partinfo>BBa_J70638 short</partinfo> | ||
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derived from K274002 | derived from K274002 | ||
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Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC | Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC | ||
| − | Suffix: | + | |
| + | Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG | ||
===References=== | ===References=== | ||
Revision as of 00:38, 19 November 2010
rbs VioA rbs VioB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1029
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3814
Illegal AgeI site found at 4010 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
there was a sequence between the stop codon of vioB and rbs of vioC in original part
Source
derived from K274002
Prefix: GCGGCCGCTTCTAGAGTTAAGGAGGTAAAAAAAATGAAACATTCTTCC
Suffix: GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGATCCTTAGGCCTCGCGGCTCAG