Difference between revisions of "Part:BBa K392036"
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<partinfo>BBa_K392036 short</partinfo> | <partinfo>BBa_K392036 short</partinfo> | ||
Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein. | Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein. | ||
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+ | See experience page for experimental data. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 01:40, 8 November 2010
endoglucanase/CBM secretion testing construct for yeast
Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein.
See experience page for experimental data.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1604
Illegal NotI site found at 2748 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2661
Illegal BamHI site found at 1797
Illegal XhoI site found at 2159
Illegal XhoI site found at 2408 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
Illegal NgoMIV site found at 1939
Illegal NgoMIV site found at 2864 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 830
Illegal BsaI site found at 1843