Difference between revisions of "Part:BBa K410000"
Biomedical (Talk | contribs) |
Biomedical (Talk | contribs) (→Usage and Biology) |
||
| Line 12: | Line 12: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | |||
| − | |||
'''Characterization''' | '''Characterization''' | ||
| Line 31: | Line 29: | ||
| − | + | <span class='h3bb'>Part Design</span> | |
| + | <partinfo>BBa_K410000</partinfo> | ||
Revision as of 18:45, 7 November 2010
Periplasmic Heat Generation
What it is:
This part consists of hybB-ompA-AOX1A.
HybB is a cold shock promoter. OmpA is a periplasmic targeting sequence, and AOX1A is a gene responsible for thermogenesis in the sacred lotus, a plant.
What it does:
Induces AOX1A expression in response to a cold shock. Here, we describe a "cold shock" as incubating E. coli between 20-30C for 10-12 hours. The part should produce a thermogenic response raising the temperature of the E. coli's immediate environment (plate or liquid cultures) above ambient temperature.
Usage and Biology
Characterization
Absolute Temperature Characterization
pSB1A3 containing biobrick part hybB-OmpA-AOX1a was transformed into E. coli BL21. Transformants were grown in 600 mL of LB media(+carbenicillin) at 37 degrees Celcius overnight. The solutions were centrifuged, the soft pellet collected, and resuspended in equivalent amount of fresh LB media(+carbenicillin). The cultures were then placed in a 10 degrees Celcius incubator for 1 hour. 75 mL were pipetted into three different insulated containers and placed in 15, 20 and 25 degrees Celcius incubators for 1 hour acclimation period. Temperatures were recorded at regular intervals using a thermistor. In addition to the pSB1A3 vector containing the biobrick part, a control (pSB1A3 vector with insert hybB-OmpA-mRFP) was also monitored. The graphs shown below illustrate absolute temperature change over time at different incubation temperatures.
The graph below illustrated absolute temperature difference from initial temperature, when t=0.
Part Design
BBa_K410000