Difference between revisions of "Part:BBa K342001:Design"

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===Design Notes===
 
===Design Notes===
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The sequence comes from the first chromosome of bacteria <i>Ralstonia eutropha H16</i> (old named <i>Alcaligenes eutrophus</i>), (reference: NC_008313), adapted to become a standard BioBrick (that is to say, without forbidden restriction sites).
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===Source===
 
===Source===
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Synthesized (M. Gene)
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Synthesized (M. Gene)<br>
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<i>phaC</i> from <i>Ralstonia eutropha H16</i><br>
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===References===
 
===References===
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“Cloning and expression in <i>Escherichia coli</i> of the <i>Alcaligenes eutrophus H16</i> Poly-beta-hydroxybutyrate biosynthetic pathway”, Steven C. Slater, William H. Voige and Douglas E. Dennis, Oct. 1988, Journal Of Bacteriology, p. 4431–4436.
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Latest revision as of 17:55, 7 November 2010

Pha C (poly-β-hydroxybutyrate polymerase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 320
    Illegal NgoMIV site found at 554
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 1145
    Illegal NgoMIV site found at 1558
    Illegal NgoMIV site found at 1625
    Illegal AgeI site found at 293
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence comes from the first chromosome of bacteria Ralstonia eutropha H16 (old named Alcaligenes eutrophus), (reference: NC_008313), adapted to become a standard BioBrick (that is to say, without forbidden restriction sites).

Source

Synthesized (M. Gene)
phaC from Ralstonia eutropha H16

References

“Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 Poly-beta-hydroxybutyrate biosynthetic pathway”, Steven C. Slater, William H. Voige and Douglas E. Dennis, Oct. 1988, Journal Of Bacteriology, p. 4431–4436.