Difference between revisions of "Part:BBa K330002"

Latest revision as of 04:37, 7 November 2010

gusA reporter gene

The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.

Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.

THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE (https://parts.igem.org/Part:BBa_K330002:Experience).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:13, 28 October 2010 (view source) Rory li (Talk \| contribs) ← Older edit		Latest revision as of 04:37, 7 November 2010 (view source) Hanson (Talk \| contribs)
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	Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.		Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.

−	THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE.	+	THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE (https://parts.igem.org/Part:BBa_K330002:Experience).