Difference between revisions of "Part:BBa I712019:Experience"

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The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J13002 :BBa_J13002]) with the addition of D-luciferin.  Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.
 
The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J13002 :BBa_J13002]) with the addition of D-luciferin.  Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.
 
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<I>Username</I>
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Enter the review inofrmation here.
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<I>theos</I>
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The Tianjin 2010 igem team confirmed that this part works well since we have used this part to construct our parts which separate the luciferase gene. Our sequencing result reveal that the quality of this part is ok.|};
  
 
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Revision as of 20:51, 6 November 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I712019

User Reviews

UNIQ01f37f87ccee29d0-partinfo-00000000-QINU

•••

theos

The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector (:BBa_J13002) with the addition of D-luciferin. Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.

;

UNIQ01f37f87ccee29d0-partinfo-00000002-QINU theos |width='60%' valign='top'| The Tianjin 2010 igem team confirmed that this part works well since we have used this part to construct our parts which separate the luciferase gene. Our sequencing result reveal that the quality of this part is ok.|};

UNIQ01f37f87ccee29d0-partinfo-00000003-QINU