Difference between revisions of "Part:BBa K310000"

Latest revision as of 15:48, 6 November 2010

micA (sRNA)

mFOLD for this sRNA

Targeted Regulation by sRNAs:micF, micA, and gadY. sRNAs expressed under lac promoter.

micA is a small non-coding RNA regulator that binds to the 5' UTR of ompA mRNA transcript via non-perfect base pairing. The primary action of micA is the translational repression of ompA by ribosome occlusion of ompA mRNA and subsequent destabalization of the transcript. micA binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex, and specifies cellular localization of sRNA-mRNA interactions.

Usage and Biology

micA sRNA is a post-transcriptional regulator. Using in conjunction with transcriptional regulators yield clear and strong bistability of target gene expression (data not shown). Endogenous to E. coli, micA regulates the expression of the outer membrane proteins in response to environmental stress conditions.

note

-8 nucleotides have been added to the 5' of this sRNA as an artifact of the RFC10 Biobrick assembly (scar coding).

-overexpression of sRNA to target mRNA yields optimal results (10 sRNA:1 mRNA)

-overexpression of hfq may affect efficacy of sRNA regulation, but pleiotropic effects observed in cell growth.

-actual biobrick is much longer than the length of the sRNA; the suffix was placed downsteam of the sRNA intrinsic terminator.








Sequence and Features